TwistDx--等溫擴增試劑盒相關文獻與應用

來源: 默瑞(上海)生物科技有限公司   2020-3-25   訪問量:1597評論(0)

 What is RPA?

  概念:重組酶聚合酶擴增(Recombinase Polymerase Amplification,RPA),被稱為是可以替代PCR的核酸檢測技術。RPA技術主要依賴于三種酶:能結合單鏈核酸(寡核苷酸引物)的重組酶、單鏈DNA結合蛋白(SSB)和鏈置換DNA聚合酶。這三種酶的混合物在常溫下也有活性,最佳反應溫度在37°C左右。

  RPA技術原理:

  重組酶與引物結合形成的蛋白-DNA復合物,能在雙鏈DNA中尋找同源序列。一旦引物定位了同源序列,就會發生鏈交換反應形成并啟動DNA合成,對模板上的目標區域進行指數式擴增。被替換的DNA鏈與SSB結合,防止進一步替換。在這個體系中,由兩個相對的引物起始一個合成事件。整個過程進行得非常快,一般可在十分鐘之內獲得可檢出水平的擴增產物。

  Who uses RPA?

  以此為基礎的TwistAmp® 核酸擴增產品,能夠在15分鐘內進行常溫下的單分子核酸檢測該技術對硬件設備的要求很低,特別適合用于體外診斷、獸醫、食品安全、生物安全、農業等領域。

RPA等溫擴增試劑盒配套試紙條發表的文獻

1、Piepenburg O., Williams C.H., Stemple D.L., Armes N.A.

DNA detection using recombination proteins. PLoS Biol 2006; 4(7): e204.

DOI:10.1371/journal.pbio.0040204https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475771/pdf/pbio.0040204.pdf

2、Saldarriaga O. A. et al., An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannis ssp. Infections. PLoS Negl. Trop. Dis. 2016; 10 (4)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845993/pdf/pntd.0004638.pdf

3、Yang Y et al., Development of an Isothermal Amplification-Based Assay for Rapid Visual Detectionof an Orf Virus. Virol. J. 2016; 13:46

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799565/pdf/12985_2016_Article_502.pdf

4、Cordray MS et al., A Paper and Plasitc Device for the Combined Isothermal Amplification and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661981/pdf/12936_2015_Article_995.pdf

5、Rosser et al., Isothermal Recombinase Polymerase Amplification (RPA) ofSchistosoma haematobium DNA and Oligochromatographic Lateral Flow Detection.Parasit Vectors 2015; 8,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559068/pdf/13071_2015_Article_1055.pdf

6、CastellanosGonzalez A et al., A Novel Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care.Am. J. Med Hyg. 2015, 93 (5), 970-975

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703275/pdf/tropmed-93-970.pdf

7、Chao CC et al., Development of Recombinase Polymerase Amplification Assays for Detectionof Orienta Tsutsugamushi or Rickettsia typhi. PLoS Negl. Trop. Dis.2015; 9 (7)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498641/pdf/pntd.0003884.pdf

8、Nair G. et al., Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop.Med. 2015; 93 (3) 591-595

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559702/pdf/tropmed-93-591.pdf

9、Rohrman B. et al., Inhibiotion of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA. Anal. Chem. 201587 (3), 1963-1967http://pubs.acs.org/doi/pdf/10.1021/ac504365v

10、Zhao G et al. Development of a recombinase polymerase amplificationcombined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis. BMC Vet Res.2018 Dec 20;14(1):412

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302395/pdf/12917_2018_Article_1703.pdf

11、Zhao G. et al., Rapid visual detection of Mycobacterium avium subsp.paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick. J Vet Sci. 2018 Mar 31;19(2):242-250.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879072/pdf/jvs-19-242.pdf

12、Qi Y. et al., Development of a rapid and visual nucleotide detection method for a Chinese epidemicstrain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test. Int J Infect Dis. 2018 May;70:42-50.https://www.ijidonline.com/article/S1201-9712(18)30062-6/pdf

13、Ahmed F. A. et al., Genome-informed diagnostics for specific and rapid detection ofPectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.Sci Rep. 2018 Oct 29;8(1):15972

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206099/pdf/41598_2018_Article_34275.pdf

14、Qi Y. et al., Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test. PLoS One. 2018Nov 26;13(11)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257923/pdf/pone.0207811.pdf

15、Wang H. et al., Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymeraseamplification combined with a lateral flow dipstick. J Fish Dis. 2018May 28https://onlinelibrary.wiley.com/doi/abs/10.1111/jfd.12808

16、Wang H. et al., Development and evaluation of serotype-specific recombinase polymeraseamplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouthdisease virus serotype A, O and Asia1. BMC Vet Res. 2018Nov 20;14(1)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245561/pdf/12917_2018_Article_1644.pdf

17、Soliman H. et al., Recombinase polymerase amplification assay combined with a lateral flowdipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferativekidney disease in salmonids. Parasit Vectors. 2018 Apr11;11(1):234.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896054/pdf/13071_2018_Article_2825.pdf

18、Soliman H. and El-Matoubli M., Rapid detection and differentiation ofcarp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761-772.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12774#accessDenialLayout

19、Zasada A. A. et al., Isothermal DNA amplification combined with lateral flow dipsticks fordetection of biothreat agents. Anal Biochem. 2018 Nov 1;560:60-66.

https://www.sciencedirect.com/science/article/pii/S0003269718307085?via%3Dihub

20、Zhao G. et al., Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida. BMC Vet. Res. 2019; 15:154

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475771/pdf/pbio.0040204.pdf

21、Li T.-T. et al., Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay. Front. Cell. Infect. Microbiol.2019; 9 1.https://www.frontiersin.org/articles/10.3389/fcimb.2019.00001/full

22、Ma Q. et al., Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid. BMC Infect. Dis.2019; 19:108

https://www.researchgate.net/publication/330863615_Development_of_a_lateral_flow_recombinase_polymerase_amplification_assay_for_rapid_and_visual_detection_of_Cryptococcus_neoformansC_gattii_in_cerebral_spinal_fluid/fulltext/5c59049792851c22a3aa5082/330863615_Development_of_ a_lateral_flow_recombinase_polymerase_amplification_assay_for_rapid_and_visual_detection_of_ Cryptococcus_neoformansC_gattii_in_cerebral_spinal_fluid.pdf?origin=publication_detail

23、Peng Y. et al., Rapid detection of Burkholderia pseudomallei with a lateral flow recombinasepolymerase amplification assay. PLoSONE 2019; 14(7)

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0213416&type=printable

24、Sullivan, T. J., Dhar, A. K., Cruz-Flores, R., & Bodnar, A. G. (2019). Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp. Scientific Reports, 9(1), 1–7. https://doi.org/10.1038/s41598-019-56170-y

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